HELPING THE OTHERS REALIZE THE ADVANTAGES OF BONGKREKIC ACID

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Subsequently, a two hundred-μl fixation buffer was utilized to fix the cells for 20 min. Right after washing the cells thrice with precooled PBS, one× intracellular staining permeabilization clean buffer containing one% DAPI (blue) was applied to mark the nuclei in the cells. 10 minutes later on, the cells were being washed three times with prec